New genetically distinct phytoplasmas and insect carriers associated with pine tree disease revealed by a survey in Curonian Spit, Lithuania
نویسندگان
چکیده
Our previous studies reported that phytoplasma was the causative agent of pine disease in Curonian Spit, Lithuania. In this study, insects from diseased trees and their adjacent areas were collected 2016 to 2019 further identify potential insect vectors spread phytoplasmas. A total 1018 phloem-feeding (order Hemiptera) identified, 98.62% which aphids (Aphididae), no known found. The results semi-nested polymerase chain reaction (PCR) using phytoplasma-specific universal primers revealed phytoplasmas detected Scots (Cinara pini (Linnaeus, 1758)), waxy grey needle pineti (Fabricius, 1781)), species-unknown aphids. Further sequence analysis virtual RFLP (restriction fragment length polymorphism) aphid-harbored strains indicated they closely related ‘Candidatus Phytoplasma pini’ (16SrXXI-A), but mainly 16SrXXI-A variants, also main identified trees. addition, three new subgroups delineated present study. Subgroups 16SrXXI-C 16SrXXI-D unveiled previously (but classification overlooked) Lithuanian strains. Subgroup 16SrXXI-E discovered newly transmission trial on these will provide insights into epidemiology pathosystem diseases, as well management.
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ژورنال
عنوان ژورنال: Canadian Journal of Forest Research
سال: 2022
ISSN: ['0045-5067', '1208-6037']
DOI: https://doi.org/10.1139/cjfr-2021-0152